![](https://downloads.microscope.healthcare.nikon.com/production/imager/mastheads/171315/lambdad-mastheadB_f076438097b2d2d85c6c3ef04742411e.jpg)
生物现象的精细捕捉
随着共聚焦显微镜和高灵敏度、大视场CMOS相机等数字化技术的发展,显微镜成像也正取得日新月异的发展。新开发的 CFI Plan Apochromat Lambda D 是一个高性能物镜系列,针对未来生命科学研究必不可少的数字解决方案进行了优化。
Lambda D 系列物镜可在 25 mm 的大视场内提供高图像质量,并在宽波长范围内进行色差校正。它们提高了定量分析的准确性,实现了高度可靠的数据采集。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/171310/Lambda-d_updated_d351a3a899683d02aaaa62f0e25095df.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/productphotos/171310/Lambda-d_updated_8ce5e64c00b8e402161599a54c13a0b1.jpg 2x)
主要特性
大视野范围内明亮清晰
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171475/lambda-d_p4_bbe0662e14923d6cbe6d9b5ea7476488.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171475/lambda-d_p4_4230e6862bf6d61f215417d6fe0d2774.jpg 2x)
在25mm FOV的边缘可捕获出色的图像质量,从而提高成像通量, 缩短拍摄时间。
通过采用新开发的高折射率玻璃并校正油浸物镜中的像场弯曲,提高了图像平整度。可完美支持在 25mm大视野的边缘区域获得明亮清晰的图像。
这有助于:
- 提高光强度测量的精度
- 在短时间内生成无缝拼接的宏观图像
- 通过单一图像获取大范围的生命现象
- 提高高内涵筛选通量
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/215155/lambdad_Lambda2a_bbe0662e14923d6cbe6d9b5ea7476488.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/215155/lambdad_Lambda2a_4230e6862bf6d61f215417d6fe0d2774.jpg 2x)
常规物镜
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/215156/lambdad_Lambda2b_bbe0662e14923d6cbe6d9b5ea7476488.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/215156/lambdad_Lambda2b_4230e6862bf6d61f215417d6fe0d2774.jpg 2x)
Lambda D 物镜
BPAE细胞、染色: GFP, MitoTracker Red, DAPI; 显微镜: AX R; 物镜: CFI Plan Apochromat Lambda D 60X Oil
视场周边的光强劣化减少
由于视野周边的光强度劣化极小,因此在定量分析中可以获得高度可靠的结果。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/206806/Lambda_1_88b2948a64184c3442c94c637595d701.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/206806/Lambda_1_0f5babf0eb519e2c150755db486c0edb.jpg 2x)
小鼠肾脏、染色:Alexa 488 WGA、Alexa 568 Phalloidin、DAPI、相机:DS-Qi2、物镜:CFI Plan Apochromat Lambda D 10X
高精度图像拼接
Lambda D 系列可高效获得高质量、无缝拼接的图像。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171447/lambda-d_4-3_c_88b2948a64184c3442c94c637595d701.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171447/lambda-d_4-3_c_0f5babf0eb519e2c150755db486c0edb.jpg 2x)
大视场提高拼接效率
由于需要更少的图像进行大图拼接,25 mm FOV 的采图效率比 22 mm FOV 高 30%。
高折射率玻璃实现均匀的亮度和画质
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171450/lambda-d_4-4_c_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171450/lambda-d_4-4_c_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
Lambda D系列采用高折射率玻璃作为油浸物镜顶端透镜的材料,以校正像场弯曲。
G2镜头采用比G1镜头折射率更高的玻璃,降低了整个物镜的佩兹伐和(Petzval sum),提高了图像平整度和视野边缘的性能。
所有波长的准确数据采集
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171456/lambdad_lambda-d_5-1_ZH_temp_bbe0662e14923d6cbe6d9b5ea7476488.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171456/lambdad_lambda-d_5-1_ZH_temp_4230e6862bf6d61f215417d6fe0d2774.jpg 2x)
Lambda D 系列涵盖了常规 VC 和 Lambda 高性能物镜的校正范围。
Lambda D采用超低色散玻璃作为镜片材质,单颗物镜即可同时校正405 nm至850 nm的宽波长范围的色差。
由于每个波长的图像平面偏差是及其细微的,多色成像的精度得到提高。核染色光强度测量可获得高度可靠的定量数据。
超低色散玻璃校正色差
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171460/lambda-d_5-2_EN2_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171460/lambda-d_5-2_EN2_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
使用具有低色散特性的玻璃,支持波长范围从405 nm开始的的色差校正。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171462/lambda-d_5-3_EN2_2022-03-07-211323_uydm_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171462/lambda-d_5-3_EN2_2022-03-07-211323_uydm_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
光的成像位置因波长而异,这是产生色差的原因。通过组合多枚超低色散玻璃,可以校正不同波长的成像位置。
对高分辨率的无尽追求
尼康采用将镜片边缘加工到最小厚度的技术,最大限度地利用穿过镜片直径周边的光线来增加数值孔径,从而获取精细结构的高分辨率成像。
获取具有高S/N的清晰荧光图像。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171471/lambda-d_high-signal-to-noise-ratio-images_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171471/lambda-d_high-signal-to-noise-ratio-images_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
小鼠小肠、染色:Alexa 488、Alexa 633、显微镜:AX R、物镜:CFI Plan Apochromat Lambda D 100X Oil
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171469/lambda-d_100x-reso-2k-ave8-z_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171469/lambda-d_100x-reso-2k-ave8-z_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
小鼠脑神经(MIP)、染色:GFP、Z范围:165.5 µm、Z步长:0.147 µm、显微镜:AX R、物镜:CFI Plan Apochromat Lambda D 100X Oil
在大视野下捕获高分辨率图像。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171476/lambda-d_p5_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171476/lambda-d_p5_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
胃壁;染色:SMA抗体染色;相机:Digital Sight 10;物镜:CFI Plan Apochromat Lambda D 40XC;图像由Nichirei Biosciences Inc提供。
实现高分辨率的边缘减薄加工技术
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171465/Lambda_3c_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171465/Lambda_3c_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
每个镜片的边缘厚度都被加工得尽可能薄,以扩大每个镜片的有效直径。
![](https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171467/lambda-d_5-5_EN2_dc1f69bd8370997ae5e62fc5649398b1.jpg 1x, https://downloads.microscope.healthcare.nikon.com/production/imager/keyfeatures/Optics/Lambda-D/171467/lambda-d_5-5_EN2_a9c6d297ff9804571714b9e6afc3483d.jpg 2x)
通过扩大镜片的有效直径,可以捕捉从物体发出的具有更大发散角度的光线,从而提高数值孔径。