图像由 Laurence Pelletier Lab, LTRI提供
A1 HD25 / A1R HD25
共聚焦显微镜
终止 Replaced by AX / AX R with NSPARC
应用笔记
Selecting the Right Objectives - Bright, Sharp Imaging of Structures down to Deep Areas
2021年6月
Spherical aberrations caused by a mismatch of refractive indices may lead to a reduction in image resolution and brightness, and are one of the key problems in imaging. In this application note, we will demonstrate the effects of spherical aberration using 3D imaging of an enteroid, which is a 3D culture system for small intestinal epithelial cells, as an example, and show how to select the appropriate immersion liquid and objective.
3D Imaging of Intestinal Organoid
2021年6月
To perform high resolution observation of the deep regions of specimens during confocal imaging, selecting the objective is very important. This application note, prepared with the cooperation of Dr. Hidenori Akutsu and Dr. Tomoyuki Kawasaki of the Department of Reproductive Medicine, Center for Regenerative Medicine, National Center for Child Health and Development, will introduce an example of 3D imaging of a small intestine organoid using two different objectives, comparing the difference in image quality between them.
Quantitative 3D Imaging of Living Organs-on-Chips with a High-Speed Point-Scanning Confocal System
2021年1月
Organs-on-chips more faithfully recapitulate the 3D architectural and functional complexity of native tissues compared to standard 2D tissue culture systems. Yet these advanced cell culture platforms present technical challenges for imaging-based applications. This Application Note demonstrates how the Nikon A1R HD25 confocal point-scanning system, CFI S Plan Fluor LWD 20XC objective and NIS-Elements software can enable rapid, deep, quantitative imaging of living cells in the Emulate Organ-Chip platform.
A1R HD25：最新的共振扫描技术让新的活细胞成像方法成为可能
2019年2月
捕捉生命系统的动态图像需要高的图像采集帧率。大标本，例如整个模式生物，还需要大视野。尼康A1R HD25共聚焦系统两者都可兼顾，结合了尼康更新的高清高速共振扫描头和前所未有的25毫米视野。该系统的表现在斑马鱼胚胎应用上已经得到验证。
建议模式:
AX / AX R with NSPARC
尼康最新的共聚焦显微镜利用基于深度学习的AI工具来简化图像采集流程并提高图像质量。快速扫描与25 mm的大视场相结合，实现了高通量成像，并具有卓越的边到边图像质量。NSPARC探测器提供超高分辨率。