Sistema de Microscopia de Super-Resolução

Specifications

N-SIM S
Lateral resolution (FWHM of beads in xy)115 nm*1 in 3D-SIM mode, 86 nm*2 in TIRF-SIM mode
Axial resolution (FWHM of beads in z)269 nm*1 in 3D-SIM mode
Image acquisition timeUp to 15 fps (TIRF-SIM/2D-SIM, 2 msec exposure time)
Reconstructed image size1024 x 1024 pixels, 2048 x 2048 pixels
Imaging modeTIRF-SIM
2D-SIM
3D-SIM (Reconstruction method: slice, stack)
Multi-color imagingUp to 6 colors
Simultaneous
multi-color imaging
Two colors
Compatible laserLU-NV series laser unit
Standard: 405 nm, 488 nm, 561 nm, 640 nm
Option: 445 nm, 514 nm
Laser combination: 405 nm/445 nm/488 nm/561 nm/647 nm
Compatible microscope

Motorized inverted microscope ECLIPSE Ti2-E

  • Perfect Focus System
  • Motorized XY stage with encoders
  • Piezo Z stage
ObjectiveCFI SR HP Plan Apochromat Lambda S 100XC Sil (NA1.35)
CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49)
CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49)
CFI SR Plan Apochromat IR 60XC WI (NA 1.27)
CFI SR Plan Apochromat IR 60XAC WI (NA 1.27)
CFI Plan Apochromat Lambda 60XC (NA 0.95)*3
CFI Plan Apochromat Lambda 40XC (NA 0.95)*3
CameraORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.)
SoftwareNIS-Elements Ar
NIS-Elements C (for Confocal Microscope A1+/A1R+)
Both require additional software modules NIS-A 6D and N-SIM Analysis
Operating conditions20 ℃ to 28 ℃ (± 1.5 ℃)

*1 These values are measured using 100 nm diameter beads excited by a 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
*2 This value is measured using 40 nm diameter beads excited by a 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
*3 Supports 2D-SIM and 3D-SIM (slice reconstruction).