Especificações
Main body | AX-FNSP | AX-FNGP |
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Optical system | Infinity optical system | |
Microscope stands | AX-FNSP Single Stand | AX-FNGP Gate Stand |
Focusing | AX-FN Focusing Nosepiece Unit Motorized coaxial coarse/fine focusing Focusing stroke: Up 13 mm/Down 2 mm*1, *2, Minimum step: 0.02 μm, Motorized escape and refocus mechanism Focal plane: 400 mm above the surface of the vibration isolated table | |
Controls | AX-FNCTL Control Box AX-FNHC Hub Controller (For controlling Focusing Nosepiece Unit, Diascopic Illumination System, Stage Joystick, Motorized Epi-fluorescence Cube Turret, Motorized Quadrocular Tilting Tube 2 and DSC Zooming Port) |
Stages | AX-FNSP | AX-FNGP |
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Adapter | AX-FNSA Stage Adapter, supporting both manual and motorized XY stages. Stage height: adjustable to 2 positions depending on sample size (400 mm/200 mm from the surface of the vibration isolated table) | |
Stage | FN-3PS2 XY stage, Cross travel 29.5 (X) x 29.5 (Y) mm, with 2 auxiliary plates AX-FNS-E Motorized XY stage, Cross travel ±34 (X) x ±27 (Y) mm |
Epi-fluorescent illuminator | AX-FNSP | AX-FNGP |
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Illumination unit | NI-FLEI-2 Epi-fluorescence attachment | |
Light source | D-LEDI Fluorescent LED Illumination System | |
Filter cube turret | 6 mountable filter cubes, shutter function
| |
Photostimulation device | AX-FNBPU Stimulation Back Port, 6 mountable filter cubes, Fluorescence imaging and simultaneous stimulation imaging can be switched |
Diascopic illuminator | AX-FNSP | AX-FNGP |
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Illumination unit | AX-FNDIA Diascopic Unit 4 filter slider attachable, Condenser holder stroke: Up 2.5 mm/Down 1.8 mm, NI-PT Polarizer Turret mountable | — |
Light source | Halogen Lamp (12V100W)
| — |
Shutter | NI-SH-E Motorized Shutter | — |
Condenser | FN-C LWD condenser, O.D. 8.2 mm, NA: 0.78 | — |
Polarizer Turret | NI-PT Polarizer Turret, Visible or infrared polarizer attachable | — |
AX-FNSP | AX-FNGP | |
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Tubes | Pupillary distance: 50-75 mm, Inclination angle: 15-35 degrees, Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100 via DSC Zooming Port
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Eyepieces (F.O.V. (mm)) | CFI 10X (22), CFI 12.5X (16), CFI 15X (14.5), CFI UW 10X (25) | |
Photodetector | AX-NEU Non-descanned EPI Upright Detector | |
Nosepieces |
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Observation methods | Brightfield, Epi-fluorescence, DIC, IR-DIC | |
Power consumption | 100W | |
Weight (approx.) | 66 kg (fully motorized fluorescence system, with diascopic illuminator) | 66 kg (fully motorized fluorescence system) |
Scan head | AX R MP |
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Type | AX-SHRM AX R MP Scan Head & Controller |
FOV | φ22 mm |
Standard image acquisition | Galvano scanner Pixel size: max. 8192 x 8192 pixels Scanning speed: max. 240 fps (512 x 16 pixels), 10 fps (512 x 512 pixels) |
High-speed image acquisition | Resonant scanner Pixel size: max. 2048 x 2048 pixels Scanning speed: max. 720 fps (2048 x 16 pixels for 2K, 1024 x 16 pixels for 1K), 30 fps (2048 x 512 pixels for 2K, 1024 x 512 pixels for 1K) |
Scan mode | Line scanning, bi-direction scanning and averaging |
Simultaneous acquisition | Max. 5 channels (including a diascopic detector channel) |
IR laser wavelength range | 700-1080 nm (1080 system), 820-1300 nm (1300 system) |
Dichroic mirror | Position: 6 |
Pinhole | 6-153 µm variable |
Zoom | 1–1000X continuously variable |
Input/output port | 2 laser input ports 2 signal output ports |
Laser for multiphoton microscopy | AX R MP |
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Single 1080 system | Mai Tai HP/eHP DeepSee, Chameleon Vision II, Axon 920 |
Dual 1080 system | Chameleon Vision II + Axon 920, Axon 920 + Axon 1064 |
Single 1300 system | InSight X3, Chameleon Discovery NX |
Dual 1300 system | InSight X3 Dual Option, Chameleon Discovery NX, Chameleon Discovery NX + Axon 920 |
Incident optics | 700-1080 nm (1080 system), 820-1300 nm (1300 system), auto alignment |
Modulation | Method: AOM (Acousto-Optic Modulator) device Control: power control, ROI exposure control |
Laser for confocal microscopy (option) | AX R MP |
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4-laser unit | 405 nm, 488 nm, 561 nm and 640 nm lasers are installed |
5-laser unit | 405 nm, 488 nm, 561 nm, 594 nm and 640 nm lasers are installed |
6-laser unit | 405 nm, 445 nm, 488 nm, 515 nm, 561 nm and 640 nm lasers are installed |
NDD for multiphoton microscopy | AX R MP |
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NDD EPI unit AX-NEI (for Ti2-E) and AX-NEU (for AX-FNSP/FNGP) | Detectable wavelength range: 400-650 nm (1080 system), 400-750 nm (1300 system) Detectors: 2 GaAsP PMTs (4 GaAsP PMTs, or 3 GaAsP PMTs + 1 multi-alkali PMT are possible by adding options) |
Visible stimulation/IR imaging (option) | AX R MP |
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Opti-Microscan Photostimulator (for AX-FNSP/AX-FNGP) | Stimulation wavelength: 405 nm, 488 nm, 561 nm; Excitation wavelength for imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system) Stimulation speed: Max. 1 ms (point stimulation), Max. 20 μs/pixel (ROI stimulation) Stimulation modes: simultaneous, sequential, manual Stimulation area: square inscribed within a 22 mm-diameter circle, stimulation ROI: arbitrary pattern, no number limit |
Diascopic detector (option) | AX R MP |
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AX-DUT-MP (for AX-FNSP/Ti2-E)*6 | Detectable wavelength range: 400-920 nm Detector: Multi-alkali PMT |
Detector for confocal / multiphoton microscopy (option) | AX R MP |
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DUX-VB detector unit | Detectable wavelength range: 400-650 nm (with IR laser), 400-750 nm (with visible laser); Detection width: 10 nm to 320 nm Maximum pixel size: 8192 x 8192 (with galvano scanner) Wavelength resolution: 5 nm, wavelength range variable in 1 nm steps Compatible with galvano and resonant scanners 2 or 4 channels (Multi-alkali PMT or GaAsP PMT options) |
DUX-ST detector unit*7 | Detectable wavelength range: 400-750 nm 2 or 4 channels (Multi-alkali PMT or GaAsP PMT options) |
NSPARC Detector Unit | Equipped with SPPC (Single Pixel Photon Counter) array detector Up to 7 barrier filters can be mounted (Mountable filter: QuadBand446/523/600/677, 452/45, 525/50, 593/46, 700/75) With galvano scanner: Can be used with X resolution of 64 to 8192 pixels, Y resolution of 2 to 8192 pixels With resonant scanner: Can be used with X resolution of 256, 512, 1024 and 2048 pixels, Y resolution of 128 to 2048 pixels |
Option | AX R MP |
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Motorized XYZ | Motorized XY stage (for AX-FNSP/FNGP/Ti2-E), High-speed piezo Z stage (for Ti2-E), High-speed piezo objective-positioning system (for AX-FNSP/FNGP) |
Nosepiece for AX-FNSP/FNGP | AX-FNTN-H CFI75 single tilting nosepiece*4 |
Software | AX R MP |
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Acquisition/analysis | Imaging software (equipped with Denoise.ai noise reduction function): NIS-Elements C or NIS-Elements C-ER |
Display/image generation | 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing |
Image format | JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS |
Application | FRAP, FLIP, FRET(option), photoactivation, 3D time-lapse imaging, multipoint time-lapse imaging, colocalization |
Control computer | AX R MP |
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OS | Windows®10 Pro 64 bit, Microsoft Windows® 11 Pro |
AX R MP | |
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Visible stimulation/IR imaging (option) | Stimulation wavelength: 405 nm, 488 nm, 561 nm Excitation wavelength for Imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system) |
Compatible microscopes | Dedicated AX-FNSP/AX-GNGP motorized upright microscope system, ECLIPSE Ti2-E motorized inverted microscope |
Z step | AX-FNSP/FNGP: 0.02 µm, Ti2-E: 0.02 µm |
Recommended installation conditions | Temperature 20 - 25˚C, ± 1˚C, air conditioning at all hours Humidity 60% RH or less (no condensation) |
*1 Based on the focus position
*2 Software controlled value
*3 DIC prism slider cannot be attached
*4 FOV 12, Usable objectives: CFI75 LWD 16X W, CFI75 Apochromat LWD 20XC W, CFI75 Apochromat 25XC W, CFI75 Apochromat 25XC W 1300
*5 Cannot be used with diascopic illlumination. The FN-MN-H cannot be used with diascopic illumination only when the 400 μm objective piezo positioner (PI) is attached.
*6 Cannot be mounted on AX-FNGP
*7 Must be used with a confocal laser.