Nikon announces the CFI Plan Apo Lambda S 60XC Silicone Immersion Objective

mai. 15, 2023

Image live three-dimensional biological specimens with high resolution and minimal aberrations.

MELVILLE, N.Y. — Nikon Instruments Inc., innovator of advanced optical microscopy solutions, has announced the release of the CFI Plan Apo Lambda S 60XC objective lens, the latest in its silicone immersion series.

With the increasing demand for live imaging of complex 3D biological specimens, such as organoids and tissue explants, the need for high quality microscope objective lenses tailored towards such applications has never been greater. Silicone oil closely matches the refractive index (RI) of living cells and tissues, allowing for deep observations with minimal optical aberrations. Furthermore, silicone oil is highly evaporation resistant, enabling long-term imaging experiments.

Objective Specifications

Numerical aperture1.30
Working distance0.30 mm
Parfocal distance60 mm
Field Number25


Cover glass thickness0.15-0.19 mm
Cover glass correction range23°C & 37°C

0.15-0.29 mm

This lens may be used together with other silicone immersion series objectives to great effect. Use the 25X or 40X silicone immersion objective for scanning over a wider field of view and then seamlessly switch to the higher magnification 60X or 100X lens for fine observation.

Zoom 5X, Pixel 0.14 μm

Small intestine enteroid (Cadherin: Alexa Fluor® 555, Nuclear: DAPI)
Images courtesy of: Dr. Yuki Yokoi, Dr. Kiminori Nakamura, and Dr. Tokiyoshi Ayabe
Innate Immunity Laboratory, Department of Cell Biological Science
Faculty of Advanced Life Science
Graduate School of Life Science, Hokkaido University

Zoom 1X, Pixel 0.29 μm