Nikon Imaging Center
King's College London
The Nikon Imaging Centre at King’s College London (NIC@KCL) is a core facility for light microscopy developed in partnership between King’s College London and Nikon. The mission of NIC@KCL is to promote innovation across a broad range of biological research fields by providing access to cutting edge microscopy and imaging equipment. NIC@KCL gives users an introduction to the latest state of the art light microscopy and imaging systems, and provides training in basic and advanced light microscopy techniques for the benefit of researchers at King's and the London research community.
Contato
NIC Director
Maddy Parsons, Ph.D.
NIC Manager
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Address
King’s College LondonGuy’s Campus
London
SE1 1UL
United Kingdom
Website
Systems Available
Upright AX R Multiphoton Gate Stand Point Scanning Confocal
Optimized for deep, gentle, fast intravital imaging, AX R Multiphoton features a gate stand delivering vast space under the objective, large field of view, high speed resonant scanning, with sensitive detectors positioned closest to the sample maximizing signal collection. Equipped with standard visible imaging and NSPARC, this setup achieves significant improvement in resolution and signal-to-noise ratio in both visible and IR imaging.
AX R Confocal with NSPARC detector and ECLIPSE Ti2-E Inverted Microscope
Nikon's AX R confocal features a 25 mm FOV, up to 8192 x 8192 pixel resolution, and is equipped with AI-based tools for simplifying acquisition and analysis. The AX R includes our high definition resonant scanner, which features a next-generation optical non-linear sampling digitizer, supporting imaging at high pixel densities of up to 2048 x 2048, and with superior image quality compared to other currently available resonant scanning systems.
The Nikon Spatial Array Confocal (NSPARC) detector utilizes an ultra-low noise detector array to collect a two-dimensional image at each scanned point. This method of image scanning microscopy (ISM) improves signal-to-noise ratio by increasing the available signal level while simultaneously allowing imaging with lower excitation power.
AX R Confocal with ECLIPSE Ti2-E Inverted Microscope
Nikon's AX R confocal features a 25 mm FOV, up to 8192 x 8192 pixel resolution, and is equipped with AI-based tools for simplifying acquisition and analysis. The AX R includes our high definition resonant scanner, which features a next-generation optical non-linear sampling digitizer, supporting imaging at high pixel densities of up to 2048 x 2048, and with superior image quality compared to other currently available resonant scanning systems.
ECLIPSE Ti2-E Inverted Microscope System with DS-Qi2 and DS50M Camera
The ECLIPSE Ti2-E Inverted research microscope features a groundbreaking 25 mm field of view, maximizing the sensor area of large-format CMOS cameras and significantly improving data throughput. The Ti2-E's unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2-E's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging. This system includes a Nikon DS50M and Qi2 monochrome cameras.
ECLIPSE Ti2-E Inverted Microscope System with DS-Qi2 and DS-Fi2 camera
The ECLIPSE Ti2-E Inverted research microscope features a groundbreaking 25 mm field of view, maximizing the sensor area of large-format CMOS cameras and significantly improving data throughput. The Ti2-E's unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2-E's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging. This system includes a Nikon DS-Fi2 color camera and a monochrome Qi2 camera.
ECLIPSE Ti2-E Widefield Inverted Microscope for Intelligent High Content Screening (HCS)
The ECLIPSE Ti2-E inverted microscope features a FOV of 25 mm with Nikon’s DS-50M CMOS camera and together with PFS increases data throughput making Ti2-E a workhorse microscope for HCS. Furthermore, a PCO Edge 4.2 camera and Lumencor Spectra III light source complement the system for wider applications. Automated experiments are set up using NIS-Elements AR software with JOBS, GA3 and NIS.AI modules.
CSU-X1 Spinning Disk Confocal with ECLIPSE Ti2-E Inverted Microscope
The Yokogawa CSU-X1 spinning disk confocal combined with Nikon’s ECLIPSE Ti2-E inverted research microscope platform provides fast, high-resolution 4D imaging of living cells with minimal effects of photo-damage. The Ti2-E microscope is equipped with Perfect Focus (PFS) to enable long time-lapse imaging without focus drift. The system is also equipped with a fast, laser-based raster-scanning device (Miniscanner) for photobleaching and photostimulation.
N-STORM/TIRF Super-Resolution System
The N-STORM provides super-resolution single molecule localization imaging on the ECLIPSE Ti2-E inverted microscope. It can reach a resolution of 20 nm laterally, 50 nm axially, and also features live-cell TIRF imaging.
A1Rsi with a Ti-E Inverted Microscope
The A1si is a powerful point scanning confocal microscope system suitable for a broad range of applications, particularly spectral resolution and rapid imaging. The A1R comes equipped with a unique hybrid scan head, incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/photoactivation and acquisition. The enhanced spectral imaging capabilities feature acquisition of a 32-channel spectral image (512 x 512 pixels) with a single scan in 0.6 second. 512 x 32-pixel images can be captured at 24 fps.
Upright ECLIPSE Ni-E A1R Point Scanning Confocal with 8 slide stage
The ECLIPSE Ni-E A1 laser scanning confocal upright microscope combined with 8 slides stage is perfectly suited for broad range of biological applications particularly high content screening. The A1R confocal is equipped with a unique hybrid scan head incorporating high speed resonant scanner and high-resolution Galvano scanner which can be used in parallel to perform bleaching or photo-activation.