超分辨率显微镜系统

应用笔记

3D Super-resolution imaging of localization of H3K27me3, Lamin AC and DNA

2020年10月

In this Application Note, we introduce examples of imaging (using the N-SIM super-resolution microscope) performed by pharmaceutical scientist Dr. Tsukasa Oikawa of the Department of Molecular Biology, Hokkaido University Graduate School of Medicine. In the absence of p53, the product of a tumor suppressor gene TP53, K27-trimethylation of histone H3 (H3K27me3) was observed to occur at the perinuclear regions during DNA replication. The researcher then wanted to know the positional relationship between H3K27me3, the nuclear membrane represented by Lamin A/C, and DNA.


结构化照明显微镜(SIM)成像与共聚焦的对比

2018年9月

沿树突的树突棘的超分辨率结构光照明显微成像SIM以前尚未在固定组织中进行,这主要是由于光散射和光学像差引起的激发光条纹图案变差。


N-SIM在核纤层超微结构定量分析中的应用

2016年10月

超分辨率结构化照明显微法(SIM)所拍摄到的细胞结构可达到通过衍射极限光学成像法(例如共聚焦显微法)所拍摄的约两倍1。在本应用说明中,我们将着重介绍Shimi等人2的研究, 他们利用尼康N-SIM系统进行定量多色3D-SIM成像,绘制并比较核纤层中核纤层蛋白异构体的分布。