Nikon Instruments Europe B.V. | Europe & Africa
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A Nikon Instruments Europe BV e todas as suas subsidiárias Europeias estão a monitorizar de perto a situação com a COVID-19 (coronavirus) e a seguir todas as normas indicadas pelas autoridades de saúde locais. Saiba mais sobre as medidas que implementamos.

 

Centro de Excelência

Washington University in St. Louis

Washington University Center for Cellular Imaging

The fundamental goal of the Washington University Center for Cellular Imaging (WUCCI) is to provide access to an integrated infrastructure of light and charged particle based cellular imaging technologies. The Center Director, and his staff provide both professional guidance and work collaboratively with Washington University researchers in assay design, sample preparation and data analysis as well as develop and apply new imaging approaches and informatics methods. The Nikon Center of Excellence within the WUCCI enables investigators to gain unprecedented insights into the dynamic behavior of single molecules and the spatial organization of cells and tissues using state-of-the-art confocal, live-cell and super-resolution microscopies, creating exciting opportunities for discovery in a broad range of basic and translational research aimed at advancing our understanding of human health and disease.

Contato

CofE Director

James Fitzpatrick, Ph.D.

Address

Washington University Center for Cellular Imaging
S Taylor Ave & McKinley Ave
St Louis, MO 63110


Systems Available

A1Rsi Resonant Scanning Confocal System

This A1Rsi resonant scanning confocal system is configured on a Ti-E inverted microscope and features a spectral detector for linear unmixing of multiple overlapping signals.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1Rsi resonant scanning confocal system
  • LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
  • A1-DUS 32-channel spectral detector
  • DU4G GaAsP 4-channel confocal detector
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

CSU-X1 Spinning Disk Confocal System

This live cell imaging system features a Yokogawa CSU-X1 spinning disk confocal scanner on Ti-E inverted microscope, as well as emission splitting optics for simultaneous two-color imaging and photostimulation devices for applications such as optogenetics.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Yokogawa CSU-X1 spinning disk confocal
  • Mightex Polygon400 DMD for patterned illumination
  • Bruker Mini-Scanner galvo-based stimulation system
  • Andor TuCam two-camera adapter for simultaneous two-color imaging
  • Andor Zyla 4.2 sCMOS camera
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

N-SIM Super-Resolution System

This system features the N-SIM super-resolution structured illumination microscopy system on a Ti-E inverted microscope, suitable for both live and fixed cell imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • N-SIM super-resolution structured illumination microscopy system
  • LUN-V 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • Andor TuCam two-camera adapter for simultaneous two-color imaging
  • Andor iXon 897 EMCCD camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

N-STORM/TIRF Super-Resolution System

The N-STORM provides super-resolution single molecule localization imaging on the Ti-E inverted microscope, and also good for TIRF imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • N-STORM super-resolution single molecule localization microscopy system
  • LUN-V high power 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Andor iXon 897 EMCCD camera
  • Andor Zyla 4.2 sCMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software