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King's College London

Contato

Nikon Imaging Center

King's College London

The Nikon Imaging Centre at King’s College London (NIC@KCL) is a core facility for light microscopy developed in partnership between King’s College London and Nikon. The mission of NIC@KCL is to promote innovation across a broad range of biological research fields by providing access to cutting edge microscopy and imaging equipment. NIC@KCL gives users an introduction to the latest state of the art light microscopy and imaging systems, and provides training in basic and advanced light microscopy techniques for the benefit of researchers at King's and the London research community.

Contato

NIC Director

Maddy Parsons, Ph.D.

NIC Manager

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Address

King’s College London
Guy’s Campus
London
SE1 1UL
United Kingdom


Systems Available

AX R Confocal with NSPARC detector and ECLIPSE Ti2-E Inverted Microscope

Nikon's AX R confocal features a 25 mm FOV, up to 8192 x 8192 pixel resolution, and is equipped with AI-based tools for simplifying acquisition and analysis. The AX R includes our high definition resonant scanner, which features a next-generation optical non-linear sampling digitizer, supporting imaging at high pixel densities of up to 2048 x 2048, and with superior image quality compared to other currently available resonant scanning systems.

The Nikon Spatial Array Confocal (NSPARC) detector utilizes an ultra-low noise detector array to collect a two-dimensional image at each scanned point. This method of image scanning microscopy (ISM) improves signal-to-noise ratio by increasing the available signal level while simultaneously allowing imaging with lower excitation power.

AX R Confocal with ECLIPSE Ti2-E Inverted Microscope

Nikon's AX R confocal features a 25 mm FOV, up to 8192 x 8192 pixel resolution, and is equipped with AI-based tools for simplifying acquisition and analysis. The AX R includes our high definition resonant scanner, which features a next-generation optical non-linear sampling digitizer, supporting imaging at high pixel densities of up to 2048 x 2048, and with superior image quality compared to other currently available resonant scanning systems.

ECLIPSE Ti2-E Inverted Microscope System with DS-Qi2 and DS50M Camera

The ECLIPSE Ti2-E Inverted research microscope features a groundbreaking 25 mm field of view, maximizing the sensor area of large-format CMOS cameras and significantly improving data throughput. The Ti2-E's unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2-E's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging. This system includes a Nikon DS50M and Qi2 monochrome cameras.

ECLIPSE Ti2-E Inverted Microscope System with DS-Qi2 and DS-Fi2 camera

The ECLIPSE Ti2-E Inverted research microscope features a groundbreaking 25 mm field of view, maximizing the sensor area of large-format CMOS cameras and significantly improving data throughput. The Ti2-E's unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2-E's unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging. This system includes a Nikon DS-Fi2 color camera and a monochrome Qi2 camera.

CSU-X1 Spinning Disk Confocal with ECLIPSE Ti2-E Inverted Microscope

The Yokogawa CSU-X1 spinning disk confocal combined with Nikon’s ECLIPSE Ti2-E inverted research microscope platform provides fast, high-resolution 4D imaging of living cells with minimal effects of photo-damage. The Ti2-E microscope is equipped with Perfect Focus (PFS) to enable long time-lapse imaging without focus drift. The system is also equipped with a fast, laser-based raster-scanning device (Miniscanner) for photobleaching and photostimulation.

N-STORM/TIRF Super-Resolution System

The N-STORM provides super-resolution single molecule localization imaging on the ECLIPSE Ti2-E inverted microscope. It can reach a resolution of 20 nm laterally, 50 nm axially, and also features live-cell TIRF imaging.

A1Rsi with a Ti-E Inverted Microscope

The A1si is a powerful point scanning confocal microscope system suitable for a broad range of applications, particularly spectral resolution and rapid imaging. The A1R comes equipped with a unique hybrid scan head, incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/photoactivation and acquisition. The enhanced spectral imaging capabilities feature acquisition of a 32-channel spectral image (512 x 512 pixels) with a single scan in 0.6 second. 512 x 32-pixel images can be captured at 24 fps.