Super-resolution microscopy of neuronal proteins
14 déc. 2020
08 h 00 EST / 13 h 00 GMT / 14 h 00 CET
Luca Colnaghi, PhD
Head of Biological Regulation Unit, Mario Negri Institute for Pharmacological Research
Super-resolution microscopy allows us to go beyond the optical limits of traditional microscopes. This technique has been particularly useful in the understanding of the physiology of synapses. In this webinar, we will describe how to study co-localization of proteins with synaptic markers at a super-resolution level. We will discuss the required controls and potential pitfalls and caveats of the approach by describing the co-localization of proteins belonging to the SUMOylation machinery with pre- and post-synaptic markers such as PSD95, synaptophysin and drebrin.