Confocal Imaging of CAR-T Cell Dynamics Using an Organ-on-a-chip Platform
Evaluation of the immune effect of CAR-T (Chimeric Antigen Receptor T) cell therapy is usually performed using a model organism, which is costly and time consuming. This application note introduces an example of building a simple 3D immune cell-mediated killing assay model using AIM Biotech’s 3D cell culture chips, and measuring the immune effects of T cells by in vitro imaging. The 3D assay model makes it easy to probe different conditions in vitro such as the cancer microenvironment and T cell regulation, and it can be customized in various ways according to the purpose of the research. This assay reproduces the more spatiotemporal dynamics of cells in vitro and enables the analysis of immune cell-mediated killing under more physiological conditions as compared to 2D models.