Notas de aplicación
The Advantages of Resonant Scanning with Ultra Short Laser Exposure Times in Live Imaging
Enteroids are an excellent tool for studying intestinal epithelial functions, such as secretion of antimicrobial peptides, α-defensins by Paneth cells in innate enteric immunity. However, enteroids are very sensitive not only to such environmental factors as temperature and humidity, but also laser exposure by laser scanning microscopes, so they must be handled with care during experiments. In this application note, we will show the advantages of conducting observations with ultra-short laser exposure times using a resonant scanner by evaluating not only photobleaching of fluorescent dyes but also intestinal epithelial cell functions focusing on Paneth cell granule secretion.