Notas de aplicación

Supercharging your NSPARC time-lapse by processing with NIS Enhance.ai

julio 2025

Membrane trafficking is a typical high-speed imaging application in the life sciences. Using cellular membrane trafficking as an example, we show that Enhance.ai, a deep learning module, can be combined with the Nikon Spatial Array confocal detector (NSPARC) to speed up time-lapse imaging. This method can acquire 2D data five times faster than 3D data, while providing a better visualization than 2D deconvolution. As a result, this method can be used to increase throughput and reduce phototoxicity. Since the trained network is not a generalizable solution for deconvolution, a training procedure is necessary. This implies that custom AI tools can be integrated with the latest detector technology for enhanced results.

Keywords: high resolution, time-lapse, organelle, ER, lysosome, AI, contact sites, deconvolution, pixel reassignment, Alzheimer’s Disease, acquisition optimization