High-speed, high-resolution 3D imaging using resonant scanner and Denoise.ai
Although resonant scanners are suitable for live cell imaging as they enable confocal imaging with high temporal resolution, in terms of image resolution, galvano scanners are superior. However, the resonant scanner of the AX R confocal microscope system can capture high-resolution images at high speed because it achieves 2K pixel resolution. Moreover, using Denoise.ai, the AI module of NIS-Elements imaging software, can remove shot noise generated by resonant scanning. As this can shorten exposure times and reduce photobleaching, it is also effective for capturing images of fixed samples. This application note introduces examples of acquisition of high-speed, high definition images using a resonant scanner together with Denoise.ai.