Notas de aplicación

Cell Proliferation Assay and Optimization of HCA Assay, Using Label-Free Live Cell Imaging

septiembre 2021

In high content analysis (HCA), which automates imaging and quantitative image analysis, it is important to perform a preliminary experiment and optimize assay conditions, such as the cell seeding numbers and drug treatment time, to match the purpose of each assay. This application note introduces a label-free cell proliferation assay using phase distribution images (Volume Contrast images) that reflect cell structures generated from brightfield images with different focal planes. This allows the HCA assay to be optimized by measuring cell numbers over time without staining living cells.