Microscopio confocal multifotón de alta definición

Specifications

A1 MP+A1R MP HD
Scan head input/output port3 laser input ports, 3 signal output ports for standard, spectral and optional detector*1
FOVSquare inscribed in a Φ18 mm circle
Image bit depth4096 gray intensity levels (12 bit)
Pinhole12 – 256 μm variable (1st image plane)
Compatible microscopesECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type)
Z stepTi2-E: 0.02 µm, FN1 stepping motor: 0.05 µm, Ni-E: 0.025 µm
OptionMotorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Laser for multiphoton microscopyA1 MP+A1R MP HD
Compatible laserMai Tai HP/eHP DeepSee, InSight DS+*2, InSight DS+ Dual Option*3, InSight X3*2, InSight X3 Dual Option*3 (Spectra-Physics), Chameleon Vision II, Chameleon Discovery*3 (Coherent)
ModulationMethod: AOM (Acousto-Optic Modulator) device
Control: power control, return mask, ROI exposure control
Incident optics700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3, auto alignment
Laser for confocal microscopy (option)A1 MP+A1R MP HD
LU-N3 3 laser unit405 nm, 488 nm, 561nm lasers are installed; built-in AOTF (Cannot used with A1-DUS spectral detector)
LU-N4/LU-N4S 4laser unit405 nm, 488 nm, 561 nm, 640 nm lasers are installed; built-in AOTF (LU-N4 cannot be used with A1-DUS spectral detector)
LU-NV Laser unitCompatible laser: 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm; built-in AOTF
NDD for multiphoton microscopyA1 MP+A1R MP HD
TypeCompatible with 1080 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1), Diascopic GaAsP NDD (for Ni-E/FN1); Detectable wavelength range 380 - 650 nm*6 Compatible with 1300 nm: Episcopic GaAsP NDD (for Ti2-E/Ni-E/FN1); Detectable wavelength range 380 - 750 nm
Detector4 PMTs (3 GaAsP PMTs and 1 Multi-Alkali PMT)
Filter cube450/50, 492, 525/50, 575/25, 610/75, 629/53
Standard fluorescence detector (option)A1 MP+A1R MP HD
Detectable wavelength range400 - 750 nm (400 - 650 nm when using IR laser)
DetectorA1-DU4-2 4 Detector Unit: 4 Multi-Alkali PMTs
A1-DUG-2 GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 Multi-Alkali PMTs
Filter cube6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 594LP, 595/50, 700/75
Diascopic detector (option)A1 MP+A1R MP HD
Detectable wavelength range440 – 645 nm
DetectorMulti-Alkali PMT
Scan headA1 MP+A1R MP HD
TypeA1-SHSM-2A1-SHRM-C
Standard image acquisitionScanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction) *7
Zoom: 1-1000x continuously variable
Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z
High-speed image acquisitionScanner: resonant scanner (X-axis, resonance frequency 7.8 kHz),
galvano scanner (Y-axis)
Pixel size: max. 1024 x 1024 pixels
Scanning speed: 15 fps (1024 x 1024 pixels), 30 fps (512 x 512 pixels), 60 fps (256 x 256 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scan mode: X-Y, X-T, X-Z
Acquisition method: High-speed image acquisition, Simultaneous photoactivation and image acquisition
IR laser wavelength range700 - 1080 nm*4,*5, 700 - 1300 nm*2,*3
Dichroic mirrorLow-angle incidence method Position: 8 Standard filter: 405/488, 405/488/561, 400-457/514/IR, 405/488/543/638, IR total reflection*2,*3, BS20/80, IR
Simultaneous stimulation optionOptional hyper selector
Visible Stimulation/Visible imaging: Stimulation by 405nm, Imaging by 488-750nm
Visible stimulation/IR imaging: Stimulation by 405nm, 488nm or 561nm (selectable by NDD dichroic mirror), Imaging by 800nm-1080nm (1080nm configuration), 820nm-1080nm (1300nm configuration)

Optional 1st dichroic mirror :High reflection mirror 405,488,561,800-1080
Spectral detector (option)A1 MP+A1R MP HD
A1-DUS spectral detector unitNumber of channels: 32
Wavelength detection range: 400 - 750 nm
Spectral image acquisition speed: 4 fps (256 x 256 pixels)
Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode)
Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps
Compatible with galvano scanner only
A1-DUVB-2 GaAsP detector unitNumber of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter
Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm
Maximum pixel size: 4096 x 4096 (CB mode/VB mode)
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps
Compatible with galvano and resonant scanners
SoftwareA1 MP+A1R MP HD
Display/image generation2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image formatJP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
ApplicationFRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computerA1 MP+A1R MP HD
OSWindows 10 Pro 64bit, English version or Japanese version OS Version 1704
Windows 7 Professional, 64bit, SP1 English version or Japanese version Windows Update KB3118401 or later
CPUIntel Xeon E5-2643v4 (3.40GHz, 6 cores, 20MB, 2400MHz) or higher
RAM16GB, 32GB or 64GB
HDD1st HP Z Turbo G2 512GB PCIe M.2 SSD, 2nd SATA 2TB
Optical DriveSuper Multi drive, up to x 16 speed or higher
GraphicsNVIDIA Quadro K620/ K2200/ K4200/ M2000/ M4000/ M5000 (PCI Express / two-screen split display supported)
Extension slotTwo PCI Express 3.0 (x16) slots (one slot to be used for graphics), One PCI Express 3.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x8 mechanical, x4 electrical) slot, One PCI Express 2.0 (x1) slot
LAN port10/100/1000 Network/Interfacex2 (for connection to controller, for connection to external LAN)
Monitor1600 x 1200 or higher resolution, dual monitor configuration recommended

*1 FCS/FCCS/FLIM is possible in combination with third-party systems.

*2 When using accessories with 1300 nm.

*3 When using accessories with 1300 nm and Dual IR.

*4 When using accessories with 1080 nm.

*5 When using accessories with 1080 nm and visible light photoactivation/IR imaging.

*6 400 - 650 nm when using diascopic NDD.

*7 Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.