Noticias
Nikon announces the CFI Plan Apo Lambda S 60XC Silicone Immersion Objective
may 15, 2023
Image live three-dimensional biological specimens with high resolution and minimal aberrations.
MELVILLE, N.Y. — Nikon Instruments Inc., innovator of advanced optical microscopy solutions, has announced the release of the CFI Plan Apo Lambda S 60XC objective lens, the latest in its silicone immersion series.
With the increasing demand for live imaging of complex 3D biological specimens, such as organoids and tissue explants, the need for high quality microscope objective lenses tailored towards such applications has never been greater. Silicone oil closely matches the refractive index (RI) of living cells and tissues, allowing for deep observations with minimal optical aberrations. Furthermore, silicone oil is highly evaporation resistant, enabling long-term imaging experiments.
Objective Specifications
| Item | Specification |
|---|---|
| Magnification | 60X |
| Numerical aperture | 1.30 |
| Working distance | 0.30 mm |
| Parfocal distance | 60 mm |
| Field Number | 25 |
| Flatness | Plan |
| Coating | Nanocrystal |
| Cover glass thickness | 0.15-0.19 mm |
| Cover glass correction range | 23°C & 37°C
0.15-0.29 mm |
This lens may be used together with other silicone immersion series objectives to great effect. Use the 25X or 40X silicone immersion objective for scanning over a wider field of view and then seamlessly switch to the higher magnification 60X or 100X lens for fine observation.
SIL25X/1.05
Zoom 5X, Pixel 0.14 μm
Small intestine enteroid (Cadherin: Alexa Fluor® 555, Nuclear: DAPI)
Images courtesy of: Dr. Yuki Yokoi, Dr. Kiminori Nakamura, and Dr. Tokiyoshi Ayabe
Innate Immunity Laboratory, Department of Cell Biological Science
Faculty of Advanced Life Science
Graduate School of Life Science, Hokkaido University
SIL60X/1.30
Zoom 1X, Pixel 0.29 μm