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Enduring Excellence - How Long-Lasting Microscopy Fuels Innovation at LIV

Dr. Christian Conze
Head of Technology Platform Light Microscopy and Image Analysis at Leibniz Institute of Virology (LIV), Hamburg

Celebrating Nikon Microscopy’s 100th Anniversary, our Enduring Excellence campaign honors researchers whose long-term use of Nikon systems showcases the sustainability and lasting value built into our instruments. In this edition, we turn to Christian Conze, Head of the Technology Platform for Light Microscopy and Image Analysis at the Leibniz Institute of Virology (LIV) Nikon Center of Excellence in Hamburg, who has relied on long‑serving Nikon microscopes throughout his research journey.

A Longstanding Relationship with Nikon Microscopes

Christian operating the AZ100 Microscope at the LIV microscopy platform.

Christian first encountered Nikon systems during his doctoral work in 2016, using an ECLIPSE C2 confocal system that had already been in service for many years. Despite its age, the system quickly proved itself reliable.  “At that time, I worked with a C2 system that still ran on what, from today’s perspective, is almost ancient EZ‑C1 software,” he recalls. “Over the years, the system was gradually modernized, received NIS‑Elements and some new hardware components, and like that it is actually still running today and being used daily at Osnabrück University. Every single paper from my time in Osnabrück (the most recent published only in 2025) features C2 scanning confocal data recorded with that device.”

His experience revealed that while software evolves, a well‑engineered optical system can remain scientifically productive for far longer than expected. “We upgraded to NIS‑Elements, and from that point on I grew to appreciate the system more and more. Nikon’s acquisition and analysis software is the main reason why I ultimately preferred Nikon systems over others at the time and still do today.”

Longevity as a Pillar of Sustainable Science

Nearly two decades on, The AZ100 Multizoom Microscope still provides robust and dependable service to the facility.

For Christian, durability and sustainability are inseparable. “Product longevity and upgradability play an extremely important role. This is especially true in the public sector, where taxpayer money is involved and responsible spending is essential.”

He sees longevity not just as a budget consideration, but as an indicator of craftsmanship: “Longevity is a strong indicator of overall product quality, since poorly made products typically have a short lifespan.”

Nikon’s modular architecture plays a major role in that sustainability. “New imaging modalities, software features, or hardware components can often be integrated without replacing the entire system. Such flexibility is crucial in a research environment where scientific questions and methodological requirements change over time.”

When Reliability Enables Breakthroughs

3D volume analysis showing plaque burden in mouse aorta (left). Image was acquired using AZ100 microscope coupled with C2+ confocal (right). Adapted from reference 1.

A standout example of longevity enabling discovery comes from cardiovascular imaging work conducted at the imaging facility at LIV. Standard confocal methods created stitching artifacts that compromised quantitative accuracy. The solution was an unconventional pairing: a C2+ scan head with an AZ100 multizoom microscope.

3D reconstruction created from 200 µm thick vibratome serial sections of Mycobacterium tuberculosis (Mtb)-infected lung. Imaged with the AZ100 microscope.

“This configuration enabled large image acquisition, eliminated stitching artifacts, and allowed reliable determination of three‑dimensional en‑face plaque volumes,” he explains. The final quantification was supported by semi‑automatic segmentation in NIS‑Elements, showing how adaptable systems can turn technical challenges into research advances.

In a new publication in EMBO Molecular Medicine, researchers turned to the long-serving BioStation IM-Q system for long-term observations of protein aggregates in live cardiomyocytes2. Highlighting that even after many years in operation, the system continued to provide the reliability needed for their research.

Built to Last — Mechanically, Optically, and Conceptually

The BioStation IM-Q system at the facility has been retrofitted into a unique solution to meet the evolving needs of its users.

At LIV, older Nikon systems continue to withstand heavy daily use, building relocations, and diverse experimental demands. “Nikon products are well suited for long‑term use… known for their robust design and high manufacturing quality, which ensures consistent performance even under intensive daily use,” Christian says.

He highlights the long-term security this provides: “Knowing that maintenance and support will remain accessible over the lifespan of the equipment gives us confidence that the instruments will remain fully operational and scientifically competitive for many years.”

A Partnership That Shapes Scientific Identity

Christian’s loyalty comes down to trust and integration. “The flexibility of Nikon systems in integrating a wide range of hardware and software features, together with excellent and reliable technical support, makes Nikon a highly valued partner for our institute.”

For him, a microscope is not just hardware, it becomes part of a scientist’s identity. When a system supports multiple research chapters across years and institutions, it forms a partnership built on both performance and longevity.

* Nikon’s C2 confocal microscope is discontinued and replaced by the AX R confocal microscope. Learn more about Nikon's 10th-generation point scanning confocal — built to last.

References

  1. Jung, C., Christiansen, S., Kaul, M. G., Koziolek, E., Reimer, R., Heeren, J., Adam, G., Heine, M., & Ittrich, H. Quantitative and qualitative estimation of atherosclerotic plaque burden in vivo at 7T MRI using Gadospin F in comparison to en face preparation evaluated in ApoE KO mice. PLoS ONE, 12(8), e0180407 (2017). DOI: 10.1371/journal.pone.0180407 
  2. Alizoti, E., Ewald, L., Parretta, S. et al. JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy. EMBO Mol Med (2026). DOI: 10.1038/s44321-026-00411-x

Note: The institutions and job titles listed with each researcher reflect their affiliation at the time of the interview.