Notas de aplicação

Measurement of mesenchymal stem cell numbers using MSC Count

junho 2019

When cell growth is evaluated over time for the purpose of selecting a batch of serum, developing culture conditions, or examining the course of the effects of drugs over time, cell numbers are periodically counted. Cells seeded in multi-well plates are dissociated and cell numbers counted at the same time every day for several days. A cell growth curve is generated from the counted results to confirm cell proliferation. Such processes are time-consuming and labor-intensive work, are difficult to standardize, require skillful techniques, and consume valuable resources including large numbers of cells, serums and media. Furthermore, even if using the cell suspension that has been adjusted by counting the number of cells contained, an error in the actual seeded number of cells is unavoidable.