Notas de aplicação
![](https://downloads.microscope.healthcare.nikon.com/production/imager/coverimages/Application-Notes/308156/2323E_Analysis_DNADamage_using_AI-driven_FullyAutomated_ECLIPSE-Ji_8f52416f9afcc73a2e17f8c24fa98668.jpg)
Analysis of DNA Damage using AI-driven fully automated ECLIPSE Ji
setembro 2023
ECLIPSE Ji with Smart Experiment software enables seamless operation from image acquisition to analysis and graph creation. Pre-trained Artificial Intelligence (AI) and pre-defined imaging procedures automatically optimize image acquisition and analysis condition settings, providing visualized data in a simple, streamlined process. γH2AX is a marker of DNA damage and DNA repair. It is known that histone protein H2AX is phosphorylated to form γH2AX at the site of DNA double-strand break (DSB), which is involved in DNA repair. This application note introduces an example of using the DNA Damage - gammaH2AX module of Smart Experiment to quantify DNA damage by measuring fluorescence intensity derived from immunofluorescence-stained gammaH2AX in the nucleus region.