Notas de aplicação
3D Super-Resolution Imaging of Glial Cells in Cleared Mouse Brain Tissue using NSPARC
outubro 2024
Glial cells play crucial physiological roles in the brain, including supporting neurons, and are implicated in neurodegeneration and lesion formation. For example, glial activation is observed in conjunction with the aggregation of misfolded proteins and neuronal cell death in neurodegenerative diseases like Alzheimer’s disease. Investigating the relationship between the progression of neurodegenerative diseases and changes in cellular microstructures requires high spatial resolution imaging of brain tissue.
This application note presents a study in which fluorescently labeled, cleared mouse brain sections were imaged in 3D with four-color super-resolution confocal laser microscopy using the AX/AX R with NSPARC. We successfully visualized the 3D morphology of synapses and the fine processes of two types of glial cells in brain tissue simultaneously at high spatial-resolution.
Keywords: NSPARC, super-resolution, glial cells, astrocytes, microglia, synapses, tissue clearing, brain tissue