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Cincinnati Children’s Hospital Medical Center

Nikon Instruments USA has partnered with Cincinnati Children’s Hospital Medical Center, to open a Nikon Center of Excellence for advanced biological imaging. The center will combine Nikon’s technical proficiency with the scientific expertise of the researchers at Cincinnati Children’s Research Foundation to further new developments in biomedical sciences that will ultimately improve child health.

Area of Expertise

This partnership provides CCRF researchers access to state-of-the art imaging equipment and Nikon technical expertise. In return, Nikon gains valuable feedback and insight from talented scientists using advanced research techniques in the diverse areas of research strength found at the CCRF. Our expertise includes phenotypic imaging and analysis of complex biological systems including developmental biology, neurobiology, stem cell biology and cancer biology. We also have a keen focus on developing and adapting new imaging techniques that can be applied to our researcher's areas of expertise.

 

联系方式

CofE Director

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Address

3333 Burnet Avenue
Cincinnati, Ohio 45229-3026
(513) 636-4425


Systems Available

N-STORM/TIRF/A1R HD Super-Resolution & Confocal System

Featuring both the N-STORM single molecule localization and A1R HD resonant scanning confocal, this system is ready for applications ranging from super-resolution to fast live-cell confocal or TIRF.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD high-definition resonant scanning confocal system
  • N-STORM super-resolution single molecule localization microscopy system
  • LUN-V high-power 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 647nm)
  • DU4G GaAsP 4-channel confocal detector
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Andor DU-897 EMCCD camera (STORM, TIRF)
  • Tokai Hit stage-top incubation system
  • Lumencor SOLA light engine
  • NIS-Elements software

A1 Point Scanning Confocal System

This correlative confocal and super-resolution system features the Nikon A1 point scanning confocal system and BioAxial CODIM conical diffraction add-on for super-resolution confocal imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1 point scanning confocal system
  • LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 647nm)
  • DU4G GaAsP 4-channel confocal detector
  • BioAxial CODIM super-resolution conical diffraction system
  • NIS-Elements software

A1 Point Scanning Confocal System

Go-to A1 point scanning confocal system for both live and fixed cell imaging applications.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1 point scanning confocal system
  • LUN-A 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • DU4G GaAsP 4-channel confocal detector
  • NIS-Elements software

A1R HD MP Resonant Scanning Multiphoton System

High-definition resonant scanning multiphoton imaging is unlocked with the A1R HD MP system, configured on an FN1 upright microscope.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD MP multiphoton system with high-definition resonant scanner
  • Coherent Chameleon Ultra II Ti:S laser unit (tunable 680-1080nm)
  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • Nosepiece-mounted Z-axis objective piezo
  • Episcopic GaAsP non-descanned detectors (NDDs)
  • DU4G GaAsP 4-channel confocal detector
  • NIS-Elements software

A1R HD Resonant Scanning Confocal System

This A1R HD resonant scanning confocal system features six laser lines for optimal excitaiton of a variety of fluorophores.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD high-definition resonant scanning confocal system
  • 6-line laser unit (405nm, 442nm, 488nm, 561nm, 640nm, 730nm)
  • DU4G GaAsP 4-channel confocal detector
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • NIS-Elements software

A1R HD Resonant Scanning Confocal System

This A1R HD resonant scanning confocal system is ready for the most challenging live cell confocal experiments, featuring both spectral detection for advanced multicolor imaging and stage-top incubation.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD high-definition resonant scanning confocal system
  • A1-DUS 32-channel spectral detector
  • 7-line laser unit (405nm, 457nm, 471nm, 488nm, 514nm, 561nm, 647nm)
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • DU4G GaAsP 4-channel confocal detector
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

A1 Point Scanning Confocal System

A1 point scanning confocal system for both live and fixed cell imaging applications.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1 point scanning confocal system
  • Lumencor SOLA light engine
  • 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • DU4G GaAsP 4-channel confocal detector
  • NIS-Elements software

Upright Widefield Microscope System

This Ni-E upright microscope features both color CCD and monochome sCMOS cameras, supporting a number of epifluorescence and transmitted light applications.

Components

  • Ni-E upright microscope
  • Andor Zyla 4.2 sCMOS camera
  • DS-Fi2 color CCD camera
  • Lumencor SOLA light engine
  • NIS-Elements software

Upright Widefield Microscope System

This Ni-E upright microscope features both color and monochome CCD cameras, supporting a number of epifluorescence and transmitted light applications.

Components

  • Ni-E upright microscope
  • Photometrics CoolSNAP HQ2 CCD camera
  • DS-Fi2 color CCD camera
  • Lumencor SPECTRA X light engine
  • NIS-Elements software

High-Speed Triggered Widefield Microscope System

This high-speed hardware-triggered widefield system is well-suited to high throughput applications.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Andor Zyla 4.2 sCMOS camera
  • DS-Fi2 color CCD camera
  • Lumencor SPECTRA X light engine
  • NIS-Elements software

High-Speed Triggered Widefield Microscope System

This high-speed hardware-triggered system based on the Ti2-E inverted microscope provides both speed and large field of view for high throughput applications.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • Andor Zyla 4.2 sCMOS camera
  • Lumencor SPECTRA X light engine
  • Lumencor LIDA light engine (RGB transmitted illuminator)
  • NIS-Elements software

Stereomicroscope System

This SMZ1500 stereomicroscope also features epifluorescence and a CMOS camera for recording observations.

Components

  • SMZ1500 stereomicroscope
  • Excelitas X-Cite Fluorescence Lamp Illuminator
  • SPOT Imaging Insight CMOS camera

Analysis Workstations

Eight offline image analysis workstations with Nikon's NIS-Elements software. NIS-Elements combines automated intelligence to microscopes, cameras, components and peripherals with powerful archiving, analysis, visualization and archiving tools. Its intuitive interface simplifies workflow and speeds up image acquisition times while providing powerful features such as image stitching, object counting and volume views.