Affiches
![](https://downloads.microscope.healthcare.nikon.com/production/imager/coverimages/Posters/184627/Eclipse_Ti2_Alignment_Poster_8f52416f9afcc73a2e17f8c24fa98668.jpg)
Köhler Illumination and Phase Contrast Alignment on the Nikon Ti2
janvier 2018
Köhler illumination is a fundamental technique for ensuring an evenly illuminated field of view for widefield observation using an optical microscope, and also the first step when aligning phase contrast optics on a microscope. This poster provides a step-by-step walkthrough of Köhler illumination and phase contrast alignment.