卓越中心
The Scripps Research Institute
Researchers at The Scripps Research Institute (TSRI) can now probe more deeply and clearly into the microscopic elements of cells with the recent opening of a new Nikon Center of Excellence on the California campus, featuring the latest in advanced imaging technology.
联系方式
CofE Director
email hidden; JavaScript is required
地址
The Scripps Research InstituteDept. of Molecular and Cellular Neuroscience
10550 N. Torrey Pines Road
DNC 210
La Jolla, CA 92037
Systems Available
AX R Point Scanning Confocal System
This system is equipped with the highly flexible AX R confocal system, which allows for fast, gentle high-resolution imaging of large tissue samples.
Components
- 8K galvo / 2K resonant imaging modes
- ECLIPSE Ti2-E inverted microscope with Perfect Focus System (PFS)
- LUA-S4 4-line laser unit (405/488/561/640 nm)
- DUX-VB4 spectral detection
- NIS-Elements software
A1R Multiphoton Confocal System
Utilizing an open frame design, this multiphoton system is ideal for deep tissue imaging, with galvo and resonance imaging capabilities and the flexibility to integrate in vivo small animal experimental setups.
Components
- 4K galvo / 1K resonant imaging modes
- Open frame upright microscope
- Removable ASI XYZ motorized stage
- Spectra-Physics Insight X3 Dual IR 1300 nm Laser
- LUN4 4-line visible laser unit (405/488/561/640 nm)
- Non-descanned detector (NDD)
- DU4G descanned detection
- NIS-Elements software
ECLIPSE Ti2 Widefield Microscope
This widefield system is equipped with a Tokai Hit incubation chamber, and is ideal for long term imaging in live cell samples.
Components
- ECLIPSE Ti2 inverted microscope
- Photometrics Iris 15 camera
- Tokai Hit incubation chamber
- Spectra III light engine
N-STORM/TIRF/A1Rsi Super-Resolution & Confocal System
Featuring both the N-STORM single molecule localization and A1R HD resonant scanning confocal, this system is ready for applications ranging from super-resolution to fast live-cell confocal or TIRF.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- A1-DUS 32-channel spectral detector
- N-STORM super-resolution single molecule localization microscopy system
- LU4A 4-line laser unit (405nm, 488nm, 561nm, 638nm)
- Mad City Labs Nano-Z series Z-axis piezo stage
- DU4 4-channel confocal detector
- Andor iXon 897 EMCCD camera
- NIS-Elements software
A1Rsi Resonant Scanning Confocal System
Using the Nikon A1Rsi resonant scanning confocal system with spectral detector, this system is made for linear unmixing of multi-labeled samples.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- A1Rsi resonant scanning confocal system
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- A1-DUS 32-channel spectral detector
- DU4G GaAsP 4-channel confocal detector
- NIS-Elements software
C2 Point Scanning Confocal System
This C2 point-scanning confocal system is configured on a 90i upright microscope and is a great option for applications requiring optical sectioning.
Components
- C2 point scanning confocal system
- LU4A 4-line laser unit (405nm, 488nm, 561nm, 638nm)
- DU3 3-channel confocal detector
- NIS-Elements software
A1R Resonant Scanning Confocal System
This A1R resonant scanning confocal system is configured on an FN1 upright microscope, and providing added flexibility for larger samples.
Components
- FN1 upright microscope
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- DU4G GaAsP 4-channel confocal detector
- NIS-Elements software
N-STORM/TIRF Super-Resolution System
While this microscope features the N-STORM localization microscopy system for super-resolution, it can also be operated as a fast live-cell TIRF microscope.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- N-STORM super-resolution single molecule localization microscopy system
- LUN-V 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- Mad City Labs Nano-Z series Z-axis piezo stage
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- World Precision Instruments AirTherm stage heater
- In Vivo Scientific CO2 controller
- NIS-Elements software