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Baylor College of Medicine

The Baylor College of Medicine Nikon Center of Excellence housed in the Center for Precision Environmental Health (CPEH) provides state-of-the-art technology to support cutting edge research converging on epigenetics, cancer biology, birth defects and environmental factors that ultimately shape disease susceptibility and outcome. The Nikon platforms will be used to leverage imaging in a variety of model systems including cell culture models, zebrafish, C. elegans and mouse/human tissues and sections.

联系方式

Director

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地址

Center for Precision Environmental Health
Baylor College of Medicine
One Baylor Plaza
Alkek Building N1317.04
Houston, TX 77030

网站

Center for Precision Environmental Health

Systems Available

N-SIM S Super-Resolution System

Double the resolution in XYZ; 100-115nm typical in XY (86nm in TIRF), 269nm in Z

Components

Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
N-SIM S super-resolution
LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
Lumencor SOLA SE II LED Fluorescence for DAPI/GFP/TXRED
NIS Elements Advanced Research with SIM Acquisition/Analysis, JOBS

Deconvolution

Widefield fluorescence, deconvolution

Components

Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Andor Zyla 4.2+ sCMOS Monochrome Camera
Lumencor Sola SE II LED Fluorescence for DAPI/GFP/TXRED/CY5
Lambda 10x, 20x, 40x, 60x oil objectives
NIS-Elements Advanced Research with 2D/3D Deconvolution and Clarify.ai

CSU-W1 Spinning Disk Confocal System

Fast acquisition confocal for optical sectioning, brightfield slide scanning, live and fixed cell

Components

Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Yokogawa CSU-W1 spinning disk confocal
405/488/561/640nm Lasers (Emission Filters: 455/50, 525/36, 605/52, 705/72)
Photometrics Prime95B sCMOS monochrome camera
Excite 110LED Fluorescence for DAPI/GFP/TXRED
Plan Apo Lambda 20x, Super Plan Fluor ELWD 20x, Plan Fluor 40x oil, 100x oil objectives
DS-Fi3 5.9MP color camera
Tokai Hit stage-top incubation system
NIS-Elements Advanced Research with extended depth of focus

TIRF/FRAP

Single molecule imaging, TIRF, FRAP, fluorescence, live and fixed cell

Components

Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
N-N-STORM illuminator with 405/488/561/647nm lasers
FRAP module
Andor iXON Ultra EMCCD monochrome camera
Plan Apo Lambda 20x, 40x, 60x oil, HP Apo TIRF 100x objective
Excite Xylis LED Fluorescence for DAPI/GFP/TXRED/CY5
Okolab H301 stage-top incubator
NIS-Elements Advanced Research with extended depth of focus, JOBS

HCA Screening

Fast, large field of view imaging in fluorescence, brightfield, plate and slide scanning/screening, live and fixed cell

Components

Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
Photometrics Prime95B sCMOS camera
DS-Fi3 5.9MP color camera
Excite Xylis LED Fluorescence for DAPI/GFP/TXRED/CY5
Plan Apo Lambda 2x, Plan Fluor 4x, 10x, Super Plan Fluor ELWD 20x, 40x objectives
Okolab H301 stage-top incubator
NIS-Elements Advanced Research with HCA, GA3, extended depth of focus, 2D/3D measurement

SMZ25 stereomicroscope

Macro-Imaging in fluorescence, developmental studies, live and fixed cell

Components

SMZ25 motorized stereomicroscope
Hamamatsu Orca Flash 4 sCMOS monochrome camera
1x and 2x Plan Apo objectives
Lumencor Sola SE II LED Fluorescence for CFP/YFP/GFP/TXRED
Tokai Hit stage-top incubation system
NIS-Elements Advanced Research