
卓越中心
Baylor College of Medicine
The Baylor College of Medicine Nikon Center of Excellence housed in the Center for Precision Environmental Health (CPEH) provides state-of-the-art technology to support cutting edge research converging on epigenetics, cancer biology, birth defects and environmental factors that ultimately shape disease susceptibility and outcome. The Nikon platforms will be used to leverage imaging in a variety of model systems including cell culture models, zebrafish, C. elegans and mouse/human tissues and sections.

联系方式
Director
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地址
Center for Precision Environmental HealthBaylor College of Medicine
One Baylor Plaza
Alkek Building N1317.04
Houston, TX 77030
Systems Available

N-SIM S Super-Resolution System
Double the resolution in XYZ; 100-115nm typical in XY (86nm in TIRF), 269nm in Z
Components
- Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- N-SIM S super-resolution
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Lumencor SOLA SE II LED Fluorescence for DAPI/GFP/TXRED
- NIS Elements Advanced Research with SIM Acquisition/Analysis, JOBS

Deconvolution
Widefield fluorescence, deconvolution
Components
- Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Andor Zyla 4.2+ sCMOS Monochrome Camera
- Lumencor Sola SE II LED Fluorescence for DAPI/GFP/TXRED/CY5
- Lambda 10x, 20x, 40x, 60x oil objectives
- NIS-Elements Advanced Research with 2D/3D Deconvolution and Clarify.ai

CSU-W1 Spinning Disk Confocal System
Fast acquisition confocal for optical sectioning, brightfield slide scanning, live and fixed cell
Components
- Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Yokogawa CSU-W1 spinning disk confocal
- 405/488/561/640nm Lasers (Emission Filters: 455/50, 525/36, 605/52, 705/72)
- Photometrics Prime95B sCMOS monochrome camera
- Excite 110LED Fluorescence for DAPI/GFP/TXRED
- Plan Apo Lambda 20x, Super Plan Fluor ELWD 20x, Plan Fluor 40x oil, 100x oil objectives
- DS-Fi3 5.9MP color camera
- Tokai Hit stage-top incubation system
- NIS-Elements Advanced Research with extended depth of focus

TIRF/FRAP
Single molecule imaging, TIRF, FRAP, fluorescence, live and fixed cell
Components
- Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- N-N-STORM illuminator with 405/488/561/647nm lasers
- FRAP module
- Andor iXON Ultra EMCCD monochrome camera
- Plan Apo Lambda 20x, 40x, 60x oil, HP Apo TIRF 100x objective
- Excite Xylis LED Fluorescence for DAPI/GFP/TXRED/CY5
- Okolab H301 stage-top incubator
- NIS-Elements Advanced Research with extended depth of focus, JOBS

HCA Screening
Fast, large field of view imaging in fluorescence, brightfield, plate and slide scanning/screening, live and fixed cell
Components
- Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Photometrics Prime95B sCMOS camera
- DS-Fi3 5.9MP color camera
- Excite Xylis LED Fluorescence for DAPI/GFP/TXRED/CY5
- Plan Apo Lambda 2x, Plan Fluor 4x, 10x, Super Plan Fluor ELWD 20x, 40x objectives
- Okolab H301 stage-top incubator
- NIS-Elements Advanced Research with HCA, GA3, extended depth of focus, 2D/3D measurement

SMZ25 Stereo Microscope
Macro-Imaging in fluorescence, developmental studies, live and fixed cell
Components
- SMZ25 motorized stereo microscope
- Hamamatsu Orca Flash 4 sCMOS monochrome camera
- 1x and 2x Plan Apo objectives
- Lumencor Sola SE II LED Fluorescence for CFP/YFP/GFP/TXRED
- Tokai Hit stage-top incubation system
- NIS-Elements Advanced Research
- 主页
- Nikon BioImaging Centers
- Baylor College of Medicine