Oil immersion operation｜Eclipse E100｜Online Guide｜Nikon Corporation Healthcare Business Unit Microscope Solutions
14.Advanced informationOil immersion operation
Oil immersion operation
Objective lens used
For the objective lens, use the oil immersion objective lens marked “Oil.”
Additionally, the black line is located on the tip of the objective lens indicates the oil immersion type objective lens.
Items to prepare
Prepare an immersion oil for immersion objective lens, lens paper, and benzene or anhydrous ethanol for cleaning after use.
Dripping immersion oil
Rotating slightly the objective which you looked for the part you want to observe, deviate it from the optical path, drip immersion oil onto the specimen (area above the condenser) carefully so that no air bubbles from forming, and put the 100X objective into the optical path slowly.
Checking for air bubbles
Check for air bubbles. Remove either the left or right eyepiece, and open the aperture diaphragm fully. Look into the binocular tube and check the pupil of objective (a bright round part). Remove any air bubbles, if you find bubble as shown in the figure.
Eliminating air bubbles
Try ① and ③ to eliminate air bubbles.
① Move the objective immerced with immersion oil back and forth by rotating the revolving nosepiece.
②If there is not enough oil, add more oil.
If the air bubbles have not been eliminated even after completing steps ① and ②, wipe off the oil and redo the oil immersion.
To avoid getting air bubbles in the oil container, turn the nozzle up vertically after dripping the oil and relax the pinched finger.
