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Center of Excellence

Aix-Marseille University

Nikon and the Neurophysiopathology Institute at Aix-Marseille University have partnered to create the Nikon Center of Excellence for Neuro-NanoImaging within its cellular imaging facility, focusing on how the latest super-resolutive technique can help in understanding brain cells and their dysfunctions.

The Center uniquely offers three different super-resolution microscopy techniques that are tailored for the subcellular investigation of neuronal function:

  • Super-resolved spinning disk (Nikon SoRa) that provides fast, gentle imaging with optical sectioning at ~150 nm resolution, ideal for live-cell imaging.
  • Structured Illumination Microscopy (Nikon N-SIM) that allows for robust, multicolor imaging with a 2x gain (~120 nm laterally) compared to diffraction-limited fluorescence microscopy.
  • Stochastic Optical Reconstruction Microscopy (Nikon N-STORM) that reaches for ~20 nm detail to dissect the nanoscale organization of macromolecular complexes directly in cells.

The Nikon Center of Excellence at INP brings these cutting-edge techniques to all neurobiology researchers at INP, in Marseille and beyond, with expert advice on how to choose the best technique for a given question and project. New sample preparation, imaging procedures, and processing methods are developed based on the Center of Excellence microscopes within the facility.

Contact

Academic Director

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Facility Manager

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Address

Institute of NeuroPhysiopathology (INP)
- UMR 7051
Faculty of Medical and Paramedical Sciences
27 Boulevard Jean Moulin
13385 Marseille Cedex 5, France


Systems Available

CSU-W1 SoRa Spinning Disk Confocal and Super-Resolution System

The CSU-W1 provides fast spinning disk confocal performance with large fields of view and deep in samples. Two cameras allow for simultaneous 2-color acquisition. In addition, the SoRa super-resolution module, based on optical pixel reassignment, can reach resolutions of 170 nm laterally, 350 nm axially with no compromise on the acquisition speed.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Yokogawa CSU-W1 spinning disk confocal with SoRa super-resolution module
  • 2x Hamamatsu FusionBT sCMOS cameras
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • OkoLab enclosure incubation system
  • NIS-Elements Advanced Research with 2D/3D Deconvolution and Clarify.ai

N-SIM Super-Resolution System

The N-SIM provides super-resolution Structured Illumination Microscopy (SIM) on the Ti2-E inverted microscope. It can reach a resolution of 120 nm laterally, 250 nm axially and is compatible with live-cell imaging.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • N-SIM S super-resolution
  • LUN-V 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Hamamatsu FusionBT sCMOS camera
  • Tokai Hit stage top incubator
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • NIS-Elements Advanced Research with 2D/3D reconstruction and Enhance.ai

N-STORM/TIRF Super-Resolution System

The N-STORM provides super-resolution single molecule localization imaging on the Ti2-E inverted microscope. It can reach a resolution of 20 nm laterally, 50 nm axially, and also features live-cell TIRF imaging.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • Motorized TIRF Illuminator Unit
  • LUN-V 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Hamamatsu FusionBT sCMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Okolab stage-top incubation system
  • NIS Elements software with N-STORM module