Webinars

Nikon Europe BV et toutes les filiales Nikon en Europe suivent de près la situation liée au COVID-19 (coronavirus) et appliquent les recommandations de chaque autorité sanitaire locale. En savoir plus sur les mesures mises en place.

 

Stay connected with the latest imaging applications, technologies and relevant topics with Nikon’s live and recorded webinars. These free events are presented by thought leaders in the imaging field and industry professionals.

Upcoming Webinars

Imaging and Quantification of Human Astrocytes in Alzheimer´s Disease Chimeras and Denoise.ai

Speakers:

  • Dr. Amaia Arranz

    Achucarro Basque Center for Neuroscience and Ikerbasque, Spain

  • Mrs. Nikky Corthout

    Light Microscopy and Imaging Network LiMoNe - Dr. Sebastian Munck. VIB BioImaging Core Leuven - Center for Brain and Disease Research

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Dr. Amaia Arranz studies Alzheimer´s disease using murine xenograft models of human neurons and glial cells exposed to disease-associated factors. Dr. Arranz aims to unravel human-specific pathways and mechanisms involved in the pathogenesis of Alzheimer´s and other neurodegenerative diseases to aid their diagnosis and treatment. In her talk, Dr. Arranz will present her research and elucidate the use of Denoise.ai in astrocyte quantification.

Mrs. Nikky Corthout is an expert microscopist dedicated to assisting researchers at the core facility of the VIB Center for brain and disease research focusing on neurobiology and brain disease. Nikky is experienced with imaging of a wide variety of samples ranging from neuronal cultures and brain sections, to entirely cleared brains,

Hosted by:

Learn More and Register

Super-resolution spinning disk confocal microscopy for life science research

Speakers:

  • Dr. Laurence Pelletier

    Professor, University of Toronto & Lunenfeld-Tanenbaum Research Institute (Sinai Health)

  • Dr. Kenneth Prehoda

    Professor, University of Oregon

  • Dan Collins

    Applications Specialist, Yokogawa Life Innovations

Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope. In this webcast, we will explore the concept of optical pixel reassignment and see how it has been applied in cell and developmental biology research. In one example, Dr. Prehoda will describe how live imaging of neural stem cells using both traditional and super-resolution imaging revealed how the plasma membrane plays a role in neurogenesis. In another example, Dr. Pelletier will discuss how the use of “smart” automated low- to super-resolution imaging pipelines can allow us to unravel the mysteries of centrosome biogenesis and function and how the molecular mechanisms underlying their function can go awry in disease states.

Hosted by:

Check back for registration information.

Past Webinars

How to revolutionize our microscope image analysis with AI

Speakers:

  • Dr. Anne-Sophie Mace

    Image Analysis Expert at the Cell and Tissue Imaging Facility at Curie Institute in Paris, France

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

How can we revolutionize our microscope image analysis?

How can we automate the extraction of quantitative parameters from our images?

Can we create a tailor-fit workflow for different imaging needs?

Dr. Anne-Sophie Mace will discuss how she manages to automate the extraction of quantitative parameters such as shape descriptors, co-localization, movements, characteristics, and others.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Hosted by:

Quantification methods in neurodegenerative diseases

Speakers:

  • Prof. Clemens Kaminski

    Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, United Kingdom

  • Dr. Lies De Groef

    Neural Circuit Development and Regeneration Research Group, Division of Animal Physiology and Neurobiology, Department of Biology, Katholiek Universiteit Leuven, in Leuven, Belgium.

  • Dr. Adam Glaser

    Department of Mechanical Engineering, University of Washington, Seattle, United States of America

  • Dr. Sebastian Munck (Webinar Host)

    VIB BioImaging Core, VIB Center for Brain & Disease Research, KU Leuven, Department of Neurosciences in Leuven, Belgium.

Register and follow the debate between key opinion leaders in neuroimaging, where they will talk about the future of quantitative methods in neurodegenerative diseases. The speakers will discuss how to use superresolution microscopy to understand diseases, how retinal models teach us about neurodegeneration, and how light-sheet microscopy and optical clearing reveal tissue composition. In addition, you will have the opportunity to ask your questions at the live Q&A session. This webinar is organized by the Nikon Center of Excellence at Katholieke Universiteit (KU) in Leuven, Belgium, and Nikon Europe BV in Amsterdam the Netherlands.

This webinar is organized by the Nikon Center of Excellence at Katholieke Universiteit (KU) in Leuven, Belgium, and Nikon Europe BV in Amsterdam the Netherlands.

Hosted by:

Can we automate measurements from 100.000 cells while maintaining their spatial relationship?

Speakers:

  • Dr. Marko Popovic

    Technical director Microscopy and Cytometry Core Facility, Amsterdam UMC location VUmc

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Is there an improvement when we apply machine learning algorithms at the center of this workflow?

Dr. Marko Popovic will discuss how combining machine learning and data analysis helps perform quantitative pathology and assess the efficacy of different cancer treatments in patients.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Recovering contrast, improving the signal-to-noise ratio, managing challenging acquisition parameters or segmentation have been challenging parts of the image analysis. You can now seamlessly automate these parts with the support of AI and accelerate your research.

Artificial Intelligence and deep learning are powerful tools that help researchers to understand processes in-vivo faster.

Hosted by:

How to build a Bioimage Analysis Workflow with multiple image analysis tools

Speakers:

  • Dr. Carlo Antonio Beretta

    Imaging Specialist, Dept. Anatomy and Cell Biology & Institute for Pharmacology, Heidelberg University

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Join our AI webinar series and learn how researchers from different fields use artificial intelligence to accelerate image analysis.

How can we investigate biological processes with lower phototoxicity in-vivo?

What can we obtain from imaging them at high temporal resolution?

Can we create a workflow that combines deep learning with standard machine learning?

Artificial Intelligence and deep learning are powerful tools that help researchers to understand processes in-vivo faster.

Dr. Carlo Antonio Beretta, from the Department for Anatomy and Cell Biology and Institute of Pharmacology at Heidelberg University, will discuss how deep learning assisted him in tackling significant obstacles that emerged while observing brain tumor invasion.

Moreover, he will discuss a workflow to accelerate the generation of precise manual ground truth data for automated 3D nuclei segmentation.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Recovering contrast, improving the signal-to-noise ratio, managing challenging acquisition parameters or segmentation have been challenging parts of the image analysis. You can now seamlessly automate these parts with the support of AI and accelerate your research.

Hosted by:

View Recording

Live imaging of host-pathogen interactions with AI

Speakers:

  • Prof. Pontus Nordenfelt

    Associate Professor at the Infection Medicine (BMC) at Lund University in Lund, Sweden

  • Dr. Simone Lepper

    European Product Manager Imaging Software & High-Content Screening microscopy

10.30 CEST

Pathogenic bacteria are armed with many mechanisms to colonize and invade human cells. Live fluorescent microscopy is a great tool for studies of these interactions but comes with several limitations in order to achieve a quantitative understanding of the pathogenic processes. Prof. Nordenfelt will describe the work in his group where they are using the AI-based clarify.ai module to enhance the analysis of host cell cytoskeletal dysregulation during host-pathogen interactions.

This webinar is created in conjunction with Bergman Labora.

Image courtesy of Oscar André and Pontus Nordenfelt.

Hosted by:

View Recording

The axonal cytoskeleton at the nanoscale

Speaker:

  • Christophe Leterrier

    Group Leader NeuroCyto lab, INP CNRS-Aix-Marseille University Marseille, France

The intricate morphology and molecular identity of axons are maintained for decades but also continuously adapt to changes in the environment and activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and mobility of axonal components. In our lab, we use super-resolution microscopy to map the nanoscale architecture actin-based structures within the axon. In the axon initial segment, a key compartment for the maintenance of neuronal polarity, we resolved a highly organized assembly encompassing the periodic actin/spectrin scaffold and its partners: ankyrin, myosin. We have also visualized new actin structures along the axon shaft: rings, hotspots and trails, and are now exploring their molecular organization and functions. For this, we develop a combination of versatile labeling, correlative live-cell/super-resolution/electron microscopy and quantitative analysis that allow for high-content, nanoscale interrogation of the axonal architecture.

Hosted by:

View Recording

Defining Functionality in Organoids

Speakers:

  • James Wells

    Chief Scientific Officer, Center for Stem Cell and Organoid Medicine (CuSTOM); Cincinnati Research Foundation Endowed Professor, Division of Developmental Biology; Director for Basic Research, Division of Endocrinology; Allen Foundation Distinguished Investigator

  • Alysson R. Muotri

    Director of the Stem Cell Program; Institute for Genomic Medicine; Dept. of Pediatrics & Cellular Molecular Medicine UCSD School of Medicine UC San Diego

  • Sally Temple

    Scientific Director of the Neural Stem Cell Institute Rensselaer, NY

Moderated by:

  • Claudia Willmes

    Trends in Molecular Medicine

Organoid technology bridges preclinical and clinical science and could solve several research questions and therapeutic challenges. Stem-cell-derived organoids have been established for multiple organs and are used for human disease modeling, host-pathogen interactions, drug discovery and toxicity testing, studying organogenesis, and developing regenerative therapies. Patient-derived organoids and technological advances that allow for targeted manipulation and growing more diverse organoids have added another layer of precision. While this emphasizes their great potential in biomedical applications and precision medicine, the rapid growth of the field also highlights its lack of cohesive definitions. In light of recent scientific breakthroughs and new use cases for organoids, it is only apt that we evaluate what a functional organoid is to exploit its full potential.

Hosted by:

View Recording

Super-resolution microscopy of neuronal proteins

Speaker:

  • Luca Colnaghi, PhD

    Head of Biological Regulation Unit, Mario Negri Institute for Pharmacological Research

Super-resolution microscopy allows us to go beyond the optical limits of traditional microscopes. This technique has been particularly useful in the understanding of the physiology of synapses. In this webinar, we will describe how to study co-localization of proteins with synaptic markers at a super-resolution level. We will discuss the required controls and potential pitfalls and caveats of the approach by describing the co-localization of proteins belonging to the SUMOylation machinery with pre- and post-synaptic markers such as PSD95, synaptophysin and drebrin.

Hosted by:

Track embryo model formation and differentiation of MSC spheroids with Nikon's BioStudio

Speakers:

  • Dr. Erik Vrij

    Postdoctoral Researcher, Maastricht University

  • Dr. Steven Vermeulen

    Postdoctoral Researcher, Maastricht University

  • Dr. Ziryan Othman

    European Product Manager, Nikon Cell Solution Business

We are excited to invite you to this webinar where we are going to discuss the available novel approaches for improving key processes in cell culturing and observation.

  • During the webinar, Nikon will introduce you the most recent cell observation systems (BioStudio-T and BioStudio-mini).
  • Learn more on the generation of MSC spheroids and the analysis of blastoid formation with the help of Nikon’s BioStudio.
  • The systems offer new insight into cellular activity as it provide long-term microscopic observation of live cells and organoids using phase contrast imaging under physiological conditions.
  • The cell observation systems work with Nikons Analysis Software which includes dedicated modules to analyze image sets for cell confluency, human pluripotent stem cell colony growth and differentiation, iPSC reprogramming efficiency, neurite outgrowth, neuronal cell/cluster count, MSC counting and migration and scratch assay.

Hosted by:

Flamingo: Putting advanced light sheet microscopy in the hands of biologists

Speaker:

  • Jan Huisken, Ph.D.

    Director of Medical Engineering

Light sheet microscopy (SPIM) has changed the field of fluorescence imaging substantially by offering a versatile technique to obtain optical sectioning in large specimens with high speed and minimal phototoxicity. Still, one of the challenges is the customization and dissemination of the technology. In this seminar Jan Huisken will give an introduction to light sheet microscopy, show a few applications in zebrafish and explain the concept and implementation of the Flamingo framework, a modular shareable light sheet microscope.

Hosted by:

View Recording

Neuronal Optical Imaging: From Cellular Landscape to Circuit Functionality

Speakers:

  • Uri Manor, Ph.D.

    Director, Waitt Advanced Biophotonics Core, Salk Institute, USA

  • Valentina Emiliani, Ph.D.

    CNRS Research Director, Wavefront-engineering Microscopy Group, Photonics Department, Institut de la Vision, Inserm, France

  • Xiaowei Zhuang, Ph.D.

    Howard Hughes Medical Institute Investigator; David B. Arnold Jr. Professor of Science; Professor of Chemistry and Chemical Biology; Professor of Physics, Harvard University

  • Rituparna Chakrabarti, Ph.D.

    Scientific Editor, iScience

In recent years, the field of neuronal imaging at the cellular and sub-cellular levels has significantly benefited from the advances in genetic engineering, development of sophisticated high-resolution instrumentation, novel probe development, enhanced computing power, and machine learning algorithms. This Cell Press webinar will showcase how these cutting-edge innovations generate valuable structure-function insight into the neuronal circuit. 

Hosted by:

Advances in Artificial Intelligence in Microscopy

Speakers:

  • Florian Jug, Ph.D

    Research Group Leader, Center for Systems Biology Dresden, Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Fondazione Human Technopole, Milano, Italy

  • Martin Weigert, Ph.D

    Group Leader, EPFL Lausanne, Switzerland

  • Christophe Zimmer, Ph.D

    Research Director, Institut Pasteur, Paris, France

The goal of this webinar is to introduce the audience to deep learning techniques for microscopy image analysis. We will give a short introduction to the foundations of deep learning for image analysis and then cover various methods for image denoising and restorations, for super-resolution microscopy, label-free prediction, and cell/object detection. After the webinar, all speakers will be available for an extensive Q&A, giving the audience the chance to ask technical as well as application specific questions. This webinar is mainly targeted to life-scientists working with microscopy image data.

Hosted by:

View Recording

Organoids as an Infection Model for SARS CoV-2

Speakers:

  • Steeve Boulant, Ph.D.

    Group Leader, Dept. of Infectious Diseases, Virology, Heidelberg University

  • Nuria Montserrat, Ph.D.

    ICREA Research Professor, Pluripotency for Organ Regeneration, Institute for Bioengineering of Catalonia

Organoids are becoming a powerful tool for studying tissue-specific host/pathogen interactions. However, their complex, three-dimensional organization can present major challenges. In this webcast, the speakers will discuss advantages, limitations and pitfalls inherent to organoids for studying host/pathogen interactions, including SARS CoV-2 infection.

Hosted by:

View Recording

Operating an Imaging Core Facility During a Pandemic

Speakers:

  • Julia Fernandez-Rodriguez, Ph.D.

    Head of the Centre for Cellular Imaging, Core Facility, Shalgrenska Academy, Univ. Gothenburg

  • Sebastian Munck, Ph.D.

    Head of the VIB BioImaging Core, VIB Center for Brain & Disease Research, KU Leuven

  • Josh Rappoport, Ph.D.

    Executive Director of Research Infrastructure, Boston College

  • Jennifer Waters, Ph.D.

    Director of Nikon Imaging Center at Harvard Medical School and Chan Zuckerberg Imaging Scientist

Operating a microscopy core facility during the current COVID-19 pandemic poses unique challenges. In this panel discussion, speakers from the US and Europe will present their varying perspectives and experiences to shed light on how core facilities and labs around the world are addressing social distancing and safety concerns for their users as institutions re-open and ramp-up research activities.

Hosted by:

View Recording

Microscope Incubators for Live Cell Imaging

Speaker:

  • Lucs Lanzaro, Ph.D

    CEO of Okolab

Which type of microscope incubator can better fit my needs: a cage incubator enclosing the whole microscope or a heated stage top chamber? Is Long-term Live Cell Imaging possible with any of these microscopy techniques: light-sheet, SIM, Super-resolution, reflected light, multiphoton, TIRF ? How can I control Oxygen levels (over that temperature, CO2 and humidity) during time-lapse experiments? Is any microscope incubator compatible with perfusion and microfluidic devices? Join the webinar to find out answers to these and more Live Cell Imaging related questions.

After registering, you will receive a confirmation email containing information about joining the webinar.

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. During this webinar we will cover exciting advances in imaging technology and how these advances are moving microscopy from R&D into screening and quality assurance.

Hosted by:

View Recording

An extraordinary microscope: expect the unexpected!

Speaker:

  • Alberto Diaspro, Ph.D.

    Department of Physics, University of Genoa, Italy Nanoscopy, Italian Institute of Technology, Italy

The meaning is related to series of advances and new methods that allow getting information at the nanoscale or preferable at the molecular scale referring to both spatial resolution or structural information.The optical microscope, from two-photon excitation to superesolved fluoresnce microscopy, becomes an extraordinary microscope. It has has tunable and flexible performances depending on the biological question and reflects the integration in a flexible way of several approaches. It is worth noting that new correlative approaches coupling optical super resolved methods with scanning probe microscopes and Mueller matrix microscopy are providing interesting developments that will be outlined. Considering all these aspects we can re-phrase the sentence related to the Galilei's "occhialino" into" Microscopium extraordinarium nominare libuit".

The hidden world revealed: in vivo study of Ca2+ dynamics in plants

Speaker:

  • Alex Costa, Ph.D.

    Dipartimento di Bioscienze, Università degli Studi Milano

Older technologies (e.g. aequorin) enabled detection of cytosolic Ca2+signatures triggered by different stimuli at low spatial resolutions. Recent developments in microscopy techniques coupled with the use of plants expressing genetically encoded fluorescent Ca2+ sensors are now revealing a hidden world where Ca2+ increases not only occur in individual, stimulated cells but propagates as waves within the plant body. The use of specific targeting sequences or tissue-specific promoters for sensor expression, is also enabling investigation into Ca2+dynamics in different subcellular compartments and in different tissues.

In the webinar, the technical aspects of the imaging tools needed to perform Ca2+ imaging in plants will be presented.

Driving cells with light-controlled topographies

Speakers:

  • Alberto Puliafito, Ph.D

    Department of Oncology, University of Torino, 10043 Orbassano, Torino, Italy. Candiolo Cancer Institute FPO-IRCCS, 10060 Candiolo, Torino, Italy.

  • Prof. Emiliano Descrovi, Ph.D.

    Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino. Department of Electronic Systems, Norwegian University of Science and Technology – NTNU, N-7491 Trondheim, Norway

In this webinar we will show how to mechanically stimulate cells adhering on a substrate. We will focus on the use of a widefield microscope to both deform the substrate where cells are growing and to monitor cell changes over time. Particular attention will be devoted to quantitative image analysis needed to characterize cell response upon mechanical stimuli. An overview of image analysis techniques such as segmentation and tracking will be presented.

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. With advances in automation, optics, hardware integration, and software, including artificial intelligence, imaging is more powerful, important, and accessible than ever.

Hosted by:

View Recording